Biotechnological Production of Preparations of Extracellular Proteases of Entomopathogenic Micromycetes

Abstract to the thesis on the topic “Biotechnological production of preparations of extracellular proteases of entomopathogenic micromycetes.” Author: Anastasia Viktorovna Koval, 4th year undergraduate student at the Faculty of Biology and Biotechnology, majoring in Cellular and Molecular Biotechnology, National Research University Higher School of Economics. The work includes: 55 pages, 4 tables, 11 drawings. Sources used - 50. Key words: micromycetes, entomopathogenic micromycetes, proteases, extracellular proteases, submerged cultivation, proteolytic activity. The object of the study is three strains of entomopathogenic micromycetes of the species Akanthomyces cf. aculeatus, Purpureocillium takamizusanense, Simplicillium wallacei. The subject of the study is extracellular proteolytic enzymes of entomopathogenic micromycetes. The purpose of the work is to select optimal conditions for cultivating promising strains of entomopathogenic micromycetes to obtain protease preparations with specificity for fibrillar and globular proteins. As part of the work, the main parameters affecting the production of extracellular proteases by micromycetes were studied: cultivation temperature, initial pH of the medium and the addition of various synthesis inducers. The activity of the obtained proteases in relation to various substrates was studied: azocasein, azocoll, fibrinogen, chromogenic substrates. Based on the results of the study, optimal cultivation conditions for the production of extracellular proteases of entomopathogenic micromycetes of the species Akanthomyces cf. aculeatus, Purpureocillium takamizusanense, Simplicillium wallacei. A preparation of extracellular proteases from the micromycete Akanthomyces cf. aculeatus, its characteristics have been studied. The results obtained can be used for the further production and use of the studied proteases in medicine and various industries.