The Effect of Mesenchymal Stem Cell Secretome on Neuronal Differentiation

Currently, there are two approaches to preclinical nerve repair: stimulation of endogenous neural precursor cells (NPCs) and transplantation of exogenous neural cells. Such exogenous cells for transplantation can be induced pluripotent stem cells, embryonic stem cells, directly reprogrammed neural precursor cells (drNPCs). The direct reprogramming method has made it possible to derive neural precursor cells from mature somatic cells without conversion to potentially harmful cells. This work represents the first attempt to investigate the effect of mesenchymal stem cell secretome on the process of neuronal differentiation of directly reprogrammed neural progenitors. The results of the study may contribute to the understanding of the mechanisms of the influence of the microenvironment of neural progenitor cells on their differentiation through the neuronal pathway. The knowledge gained will be relevant to the scientific community working in the fields of regenerative medicine, neurobiology and cell therapy. These data can be used to develop innovative approaches to the treatment of diseases of the central nervous system.