RNA-POLYMERASE III SYNTHETIC PROMOTER DESIGN

In the last few years, RNA interference-based drugs have successfully completed clinical trials and entered the market. The major problem of such drugs is their efficient delivery development to provide safe and targeted therapy. The creation of drugs based on vectors with shRNA and a tissue-specific promoter for RNA polymerase III is a way to solve this problem. This approach will reduce the risk of side effects, increase the stability and accuracy of RNA interference-based drugs. The tissue-specificity of the RNA polymerase III promoter is not known. However, the structure of this promoter is similar to the structure of the RNA polymerase II promoter, which suggests that there are similar mechanisms of interaction between transcription factors, and therefore it is possible to use the tissue specificity data of these factors for the RNA polymerase III promoter. In order to develop tissue-specific RNA polymerase III promoters, this study provides a tool to map and optimize existing RNA polymerase III promoters and to design a promoter sequence with a given tissue specificity. Keywords: RNA interference, RNA polymerase III, promoter, transcription factors, tissue specificity.