Evgeny Knyazev
- Associate Professor:Faculty of Biology and Biotechnology / Joint Department with RAS Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
- Laboratory Head:Faculty of Biology and Biotechnology / Laboratory of Molecular Physiology
- Evgeny Knyazev has been at HSE University since 2019.
Education and Degrees
- 2016
Candidate of Sciences* (PhD) in Oncology
Thesis Title: MicroRNA markers for minimally invasive diagnosis of prostate cancer - 2013
Degree in Medicine
Lomonosov Moscow State University, Faculty of Medicine
According to the International Standard Classification of Education (ISCED) 2011, Candidate of Sciences belongs to ISCED level 8 - "doctoral or equivalent", together with PhD, DPhil, D.Lit, D.Sc, LL.D, Doctorate or similar. Candidate of Sciences allows its holders to reach the level of the Associate Professor.
Continuing education / Professional retraining / Internships / Study abroad experience
2015-2016, Moscow State University of Medicine and Dentistry named after A. I. Evdokimov, Faculty of Additional Professional Education, an internship in Clinical Pathology and Laboratory Diagnostics
Awards and Accomplishments
Winner of the HSE University Best Russian Research Paper Competition – 2023
Young Faculty Support Programme (Group of Young Academic Professionals)
Category "New Lecturers" (2024)
Courses (2023/2024)
- 3rd generation Sequencing on Oxford Nanopore (Mago-Lego; 3, 4 module)Rus
- Biotechnologies: genetic engineering (Mago-Lego; 3, 4 module)Rus
- Fundamentals of statistics. Part 2 (Mago-Lego; 3, 4 module)Rus
- Introduction to NGS (Mago-Lego; 3, 4 module)Rus
- Project Seminar (Bachelor’s programme; Faculty of Biology and Biotechnology; 3 year, 3 module)Rus
- Past Courses
Courses (2022/2023)
Courses (2021/2022)
- Biochemistry (Bachelor’s programme; Faculty of Biology and Biotechnology; 2 year, 1, 2 module)Rus
- Strategic thinking in the bioeconomy (Master’s programme; Faculty of Biology and Biotechnology; 1 year, 1, 2 module)Rus
20243
- Article Vladimir Staroverov, Alexei Galatenko, Evgeny Knyazev, Alexander Tonevitsky. Mathematical model explains differences in Omicron and Delta SARS-CoV-2 dynamics in Caco-2 and Calu-3 cells // PeerJ. 2024. Vol. 12. Article e16964. doi
- Article Vishnyakova P., Gantsova E., Kiseleva V., Lazarev D., Knyazev E., Poltavets A., Iskusnykh M., Muminova K., Potapova A., Khodzhayeva Z., Elchaninov A., Fatkhudinov T., Sukhikh G. MicroRNA miR-27a as a possible regulator of anti-inflammatory macrophage phenotype in preeclamptic placenta // Placenta. 2024. Vol. 145. P. 151-161. doi
- Article Долотказин Д. Р., Аверинская Д. А., Князев Е. Н., Райгородская М. П., Колодеева О. Е., Колодеева О. Е., Шкурников М. Ю., Алексеев Б. Я. Оценка уровня miR-21-5p, miR-451a и miR-144-3p в моче при дифференциальной диагностике локализованного рака предстательной железы // Онкоурология. 2024. Т. 20. № 1. С. 36-43. doi
20235
- Article Knyazev E. N., Kalinin R. S., Abrikosova V. A., Mokrushina Y. A., Tonevitskaya S. A. KDM5 Family Demethylase Inhibitor KDOAM-25 Reduces Entry of SARS-CoV-2 Pseudotyped Viral Particles into Cells // Bulletin of Experimental Biology and Medicine. 2023. Vol. 175. No. 1. P. 150-156. doi
- Chapter Якубец Д. А., Маркина Е. А., Князев Е. Н., Буравкова Л. Б. Изменения транскриптома легких мышей после 30-суточного космического полета // В кн.: XLVII Академические чтения по космонавтике, посвященные памяти академика С.П. Королёва и других выдающихся отечественных ученых — пионеров освоения космического пространства Т. 4. М. : МГТУ им. Н. Э. Баумана, 2023. С. 48-49.
- Article Князев Е. Н., Калинин Р. С., Абрикосова В. А., Мокрушина Ю. А., Тоневицкая С. А. Ингибитор деметилаз семейства KDM5 — KDOAM-25 — снижает проникновение псевдотипированных вирусных частиц SARS-CoV-2 в клетки // Клеточные технологии в биологии и медицине. 2023. № 1. С. 40-46. doi
- Book Князев Е. Н., Чеботарева О. Е. Профессиональная учебная практика: I курс : Учебно-методическое пособие для студентов и преподавателей - 2-е изд., испр. / Рук.: Е. Н. Князев. 2-е изд., испр.. М. : Издательский дом НИУ ВШЭ, 2023. doi
- Book Профессиональная учебная практика: I курс: Учебно-методическое пособие для студентов и преподавателей / Рук.: Е. Н. Князев; сост.: Е. Н. Князев, О. Е. Чеботарева. М. : Издательский дом НИУ ВШЭ, 2023. doi
20214
- Article Knyazev E. N., Paul S. Yu., Tonevitsky A. G. Chemical Induction of Trophoblast Hypoxia by Cobalt Chloride Leads to Increased Expression of DDIT3 // Doklady Biochemistry and Biophysics. 2021. Vol. 499. No. 1. P. 251-256. doi
- Article Knyazev E., Nersisyan S., Tonevitsky A. Endocytosis and Transcytosis of SARS-CoV-2 Across the Intestinal Epithelium and Other Tissue Barriers // Frontiers in Immunology. 2021. Vol. 12. Article 636966. doi
- Article Knyazev E., Maltseva D., Raigorodskaya M., Shkurnikov M. HIF-Dependent NFATC1 Activation Upregulates ITGA5 and PLAUR in Intestinal Epithelium in Inflammatory Bowel Disease // Frontiers in Genetics. 2021. Vol. 12. Article 791640. doi
- Article Knyazev E. N., Paul S. Yu. Levels of miR-374 increase in BeWo b30 cells exposed to hypoxia // Bulletin of Russian State Medical University. 2021. No. 2. P. 11-17. doi
20206
- Article Shkurnikov M. Yu., Nersisyan S. A., Osepyan A. S., Maltseva D. V., Knyazev E. N. Differences in the Drosha and Dicer Cleavage Profiles in Colorectal Cancer and Normal Colon Tissue Samples // Doklady Biochemistry and Biophysics. 2020. Vol. 493. No. 1. P. 208-210. doi
- Article S. A. Nersisyan, M. Yu. Shkurnikov, E. N. Knyazev. Factors Involved in miRNA Processing Change Its Expression Level during Imitation of Hypoxia in BeWo b30 Cells // Doklady Biochemistry and Biophysics. 2020. Vol. 493. No. 1. P. 205-207. doi
- Article Nersisyan S., Shkurnikov M., Turchinovich A., Knyazev E., Tonevitsky A. Integrative analysis of miRNA and mRNA sequencing data reveals potential regulatory mechanisms of ACE2 and TMPRSS2 // Plos One. 2020. Vol. 15. No. 7. P. e0235987. doi
- Article Maltseva D. V., Raigorodskaya M. P., Zgoda V. G., Tonevitsky E. A., Knyazev E. N. Intracellular Transport of Ribosome-Inactivating Proteins Depends on Annexin 13 // Doklady Biochemistry and Biophysics. 2020. Vol. 494. No. 1. P. 219-221. doi
- Article Maltseva D., Raygorodskaya М., Knyazev E., Zgoda V., Tikhonova O., Zaidi S., Nikulin S., Baranova A., Turchinovich A., Rodin S., Tonevitsky A. Knockdown of the α5 Laminin Chain Affects Differentiation of Colorectal Cancer Cells and Their Sensitivity to Chemotherapy // Biochimie. 2020. Vol. 174. P. 107-116. doi
- Article Maltseva D. V., Raigorodskaya M. P., Tikhonova O. V., Knyazev E. N., Tonevitsky E. A. Relationship between the Expression Level of PSMD11 and Other Proteasome Proteins with the Activity of Ricin and Viscumin // Doklady Biochemistry and Biophysics. 2020. Vol. 493. No. 1. P. 198-200. doi
20197
- Article Nikulin S., Knyazev E., Gerasimenko T., Gazizov I., Petrov V., Kindeeva O., Sakharov D. Application of impedance spectroscopy for analysis of BeWo clone b30 human choriocarcinoma cell line // Placenta. 2019. Vol. 83. P. e21. doi
- Article Shkurnikov M. Yu., Knyazev E. N., Fomicheva K. A., Kasatkin M. S., Arkov V. V., Makarova Yu. A. Detection of Low-Abundant MicroRNAs with Hybridization Microchips // Bulletin of Experimental Biology and Medicine. 2019. Vol. 166. No. 6. P. 788-792. doi
- Article Knyazev E., Zakharova G., Astakhova L., Tsypina I., Tonevitsky A., Sukhikh G. Metabolic Reprogramming of Trophoblast Cells in Response to Hypoxia // Bulletin of Experimental Biology and Medicine. 2019. Vol. 166. No. 3. P. 321-325. doi
- Article Knyazev E. N., Petrov V., Gazizov I., Gerasimenko T. N., Tsypina I. M., Tonevitsky A. G., Sukhikh G. T. Oxyquinoline-Dependent Changes in Claudin-Encoding Genes Contribute to Impairment of the Barrier Function of the Trophoblast Monolayer // Bulletin of Experimental Biology and Medicine. 2019. Vol. 166. No. 3. P. 369-372. doi
- Article Knyazev E., Khristichenko A., Maltseva D., Gerasimenko T., Kindeeva O., Petrov V., Sakharov D. Placenta-on-a-chip model for assessing the transport and toxicity of xenobiotics in vitro // Placenta. 2019. Vol. 83. P. e59-e60. doi
- Article Sakharov D., Maltseva D., Knyazev E., Nikulin S., Poloznikov A., Shilin S., Baranova A., Tsypina I., Tonevitsky A. Towards embedding Caco-2 model of gut interface in a microfluidic device to enable multi-organ models for systems biology // BMC Systems Biology. 2019. Vol. 13. No. 19. P. 3-11. doi
- Article Knyazev E. N., Nikulin S. V., Khristichenko A. Y., Gerasimenko T. N., Kindeeva O. V., Petrov V. A., Belyakova G. A., Maltseva D. V. Transport and toxicity of 5-fluorouracil, doxorubicin, and cyclophosphamide in in vitro placental barrier model based on BeWo b30 cells // Russian Chemical Bulletin. 2019. Vol. 68. No. 12. P. 2344-2349. doi
20183
- Article Ernst A., Putscher A., Samatov T. R., Suling A., Galatenko, V. V., Shkurnikov, M. Y., Knyazev, E. N., Tonevitsky, A. G., Haalck T., Lange T., Schröder-Schwarz J., Riecken K., Schumacher U., Wicklein D. Knockdown of L1CAM significantly reduces metastasis in a xenograft model of human melanoma: L1CAM is a potential target for anti-melanoma therapy // Plos One. 2018. Vol. 13. No. 2. Article e0192525. doi
- Article Knyazev E., Poloznikov A., Maltseva D., Khristichenko A. Oxyquinoline derivative activates HIF-1 and increases transepithelial resistance of BeWo b30 monolayer // Placenta. 2018. Vol. 69. P. e47. doi
- Article Князев Е. Н., Захарова Г. С., Астахова Л. А., Цыпина И. М., Тоневицкий А. Г. Метаболическое перепрограммирование трофобласта при моделировании ответа на гипоксию // Бюллетень экспериментальной биологии и медицины. 2018. Т. 9. № 166. С. 290-295. doi
20171
Conferences
- 2019
International Federation of Placenta Associations 2019 (IFPA2019); 8th Latin American Symposium on Maternal-Fetal Interaction and Placenta (VIII SLIMP), Buenos Aires, Argentina (Buenos Aires). Presentation: Placenta-on-a-chip model for assessing the transport and toxicity of xenobiotics in vitro
International Federation of Placenta Associations 2019 (IFPA2019); 8th Latin American Symposium on Maternal-Fetal Interaction and Placenta (VIII SLIMP), Buenos Aires, Argentina (Buenos Aires). Presentation: Application of impedance spectroscopy for analysis of BeWo clone b30 human choriocarcinoma cell line
- CTR Annual Conference 2019 (Cambridge). Presentation: Placenta-on-a-chip model for the assessment of drug transport and toxicity
22st European Congress on Alternatives to Animal Testing, 19th Annual Congress of EUSAAT, Linz, Austria (Linz). Presentation: Placenta-on-a-chip model for assessing the transport and toxicity of xenobiotics in vitro
Второй международный форум онкологии и радиологии (Москва). Presentation: Модель плаценты-на-чипе in vitro для оценки транспорта и токсичности химиотерапевтических препаратов
V Всероссийская Конференция по молекулярной онкологии (Москва). Presentation: Модель плаценты-на-чипе in vitro для оценки транспорта и токсичности химиотерапевтических препаратов
Grants
2020-2022 "Coronavirus entry into cells and intracellular transport in the microfluidic model of the intestinal barrier" (RFBR Grant 20-04-60399 ) - Participant
2019-2021 "The role of miRNAs in the placenta-on-a-chip model in simulated hypoxia" (Russian Science Foundation Grant 19-74-00145) - Project Manager
2019-2021 "Transcytosis of exogenous miRNAs in a microfluidic model of the human intestinal barrier" (RFBR Grant 20-34-70092) - Project Manager
2019-2020 "Study of bacterial adhesion in the microfluidic model of the human intestinal barrier" (contract 05.617.21.0056, Ministry of Science and Higher Education of the Russian Federation) - Participant
2017-2019 “Microphysiological model of human placental barrier: Placenta-on-a-chip” (contract 14.588.21.0007, Ministry of Science and Higher Education of the Russian Federation) - Participant
2014-2016 “Development of the analytical assay based on microRNA level in blood plasma for minimally invasive diagnostics of prostate cancer” (contract 14.579.21.0054, Ministry of Education and Science of the Russian Federation) - Participant
Employment history
Dec 2019 — Present: Faculty of Biology and Biotechnology, Head of Laboratory of Molecular Physiology, Associate Professor
May 2019 — Present: Laboratory of Microfluidic Technologies for Biomedicine, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Senior Research Fellow
Feb 2013 — Sep 2023: Translation Technology Center (formerly BioClinicum Laboratory), CEO
Nov 2022 — Jun 2023: Faculty of Biomedical Engineering, Bauman Moscow State Technical University, Associate Professor
Aug 2021 — Feb 2022: Filatov City Clinical Hospital No. 15, Physician of Clinical Laboratory Diagnostics
Feb 2019 — Aug 2019: National Medical Research Center for Therapy and Preventive Medicine, Senior Research Fellow
Sep 2013 — Jun 2015: Laboratory of Cell Physiology, Institute of Biomedical Problems, Research Fellow
Feb 2012 — Jan 2013: Science and Technology Center "Cell Technologies", Research Assistant
The research from the laboratory was presented at the second Drug Developers Summit "Sirius.Biotech"
As part of the special session "Study of Safety and Biomarkers of New Drugs," a report was given on "The Use of the 'Human-on-a-Chip' Platform in Drug Development"
Evgeny Knyazev, Head of the Laboratory of Molecular Physiology, Took Part in the School of Scientific Leaders in Sochi
From November 26 to December 1, the Scientific Leaders' Offsite School and the III Congress of Young Scientists took place in Sochi and the Sirius Park of Science and Art. Evgeny Knyazev, a member of the Faculty of Biology and Biotechnology at HSE University, participated in these events.