Бакалавриат
2023/2024
Сигнальные пути в клетках
Статус:
Курс обязательный (Клеточная и молекулярная биотехнология)
Направление:
06.03.01. Биология
Кто читает:
Базовая кафедра Института биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова РАН
Где читается:
Факультет биологии и биотехнологии
Когда читается:
4-й курс, 1, 2 модуль
Формат изучения:
без онлайн-курса
Охват аудитории:
для своего кампуса
Преподаватели:
Горенкова Наталия Александровна
Язык:
английский
Кредиты:
4
Контактные часы:
28
Course Syllabus
Abstract
Overview of the subject
The life of a multicellular organisms depends on communication between its cells, which is controlled by complex cross-talk between internal and external signals. Regulation of cell activity and homeostasis, e.g. growth, division, metabolism as well as cell death, depends on the signal received. Understanding the basic molecular mechanisms of cellular signals transduction and transmission is essential for being able to manipulate with cells in vitro and in vivo, and developing any new biotechnological and biomedical applications and therapies. The course on “Cell Signaling Cascades” consists of 14 lectures. Lectures will be based on basic knowledge of biochemistry, cell and molecular biology, immunology. The concepts learnt during this course of “Cell Signalling Cascades” will be used during other disciplines like “Biotechnology and bioengineering”, “Regulation of cell cycle and gene transcription” and “Molecular oncology”. The course is intended for undergraduate 3rd year students and is offered in English in the 1st and 2nd modules.
Learning Objectives
- Educational concept and goals of the course 1. The subject is concerned on delivery of knowledge and development of understanding in basic molecular mechanisms of signal transduction pathways related to cell growth, development, stress, and also the consequences of inappropriate signaling as cancer and other abnormalities. 2. By the end of the course students should be able to easily manipulate with their systemic knowledge and terminology of the cell communication machinery and pathology-related signaling cascades, in case they wish to progress further to the area of synthetic biology, — to try their skills in synthesis of some new biological systems or modifying the existing ones in therapeutic purposes.
Expected Learning Outcomes
- The student can describe the basic fundamentals of cell signaling, its role and place in the context of main biological processes in humans
- Being able to manipulate by the modern terms of different building blocks of a signaling cascade, like modules, scaffold proteins, adaptor proteins, etc.
- Understand cellular signaling pathways that control cell growth and metabolism. Understand how cell signaling defects lead to diseases development
- Understand major types of signalling molecules, their receptors and intracellular signalling pathways.
- Describe the mechanisms by which different receptors may be activated by their respective ligands, using specific terminology of ligand affinity, receptor binding sites, etc
- Describe approaches, e.g. Western blotting, pull-down assays, and immunoprecipitation that are used to study cell signaling pathways.
- Understand the complexity, integration and intercrossing of main signaling cascades and thereby regulation of an organism functionality
- Understand the basic fundamentals of cell signaling, its role and place in the context of main biological processes in humans.
Course Contents
- Topic 1. General principles of signal transduction 1
- Topic 2. General principles of signal transduction 2.
- Topic 3. Membrane G-Coupled Protein Receptors 1
- Topic 4. Membrane G-Coupled Protein Receptors 2
- Topic 5. Intracellular Receptors and signalling.
- Topic 6. PI3K/AKT pathway.
- Topic 7. Tyrosine kinases receptors
- Topic 8. Tyrosine kinases receptors
- Topic 9. The Toll-like Receptor 4 and signalling through ubiquitylation
- Topic 10. Chemical neurotransmission in CNS: Ionotropic and Metabotropic Receptrs.
- Topic 11. Chemical neurotransmission in CNS: Ionotropic and Metabotropic Receptrs.
- Topic 12. Signaling during inflammation.
- Topic 13. Signalling cascades used during growth, differentiation and proliferation.
- Topic 14. Dysregulated signaling cascades in cancer cells.
